siRNA misc links

http://www.nature.com/cgt/journal/v13/n9/abs/7700931a.html

http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T3C-4J2KT4G-2&_user=10&_coverDate=06%2F25%2F2006&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=7f3a0e8a4ed26a4a6722ac751e72a0a8

http://www.nature.com/mt/journal/v13/n3/abs/mt200661a.html

http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6W7J-4HJRRWW-2&_user=10&_coverDate=01%2F31%2F2006&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=17ff20741ee9a9b25f5aac65acff342f

http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSN-4KH4DBN-4&_user=10&_coverDate=07%2F28%2F2006&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=759a4a2bfbd2a32cc3ce6e3e6f199f3d

http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=PubMed&list_uids=16629382&dopt=Citation

http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T64-4J853YK-B&_user=10&_coverDate=01%2F31%2F2006&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=6089df0f55fcf250a1baaf41b9a03a21

http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=PubMed&list_uids=16336204&dopt=Citation

http://www.nature.com/nrd/journal/v3/n4/full/nrd1345.html

http://www.nature.com/focus/rnai/index.html

siRNA—Getting the message out

http://www.sciencedirect.com.libproxy1.nus.edu.sg/science?_ob=ArticleURL&_udi=B6T25-4J4402J-1&_user=111989&_coverDate=04%2F30%2F2006&_rdoc=1&_fmt=summary&_orig=search&_cdi=4909&_sort=d&_docanchor=&view=c&_acct=C000008700&_version=1&_urlVersion=0&_userid=111989&md5=df049e1c21bc0bd3a09872d65bf78182

Abstract

The recent observation that potent and sequence-specific gene silencing by injection of double-stranded RNA (dsRNA) has sparked the phenomenon known as “RNA interference” (RNAi) and has enabled the gene-specific knockdown of drug transport proteins and metabolizing enzymes. The application of small interfering RNAs (siRNAs) is broad and the potential for use as research tools is now well established in vitro. In vivo use is still a challenge that is primarily focused on the difficulty of delivering siRNAs to target cells. The potential use of siRNAs as therapeutic agents is also exciting and holds great promise for future. For the study of drug transporter function in absorption, distribution, metabolism and excretion (ADME) and in the treatment of diseases, siRNA offers a way to gather interpretable mechanistic data—a distinct advantage over the use of “specific” chemical inhibitors. This mini review provides background information on siRNA as well as examples of the use of siRNA as applied to drug transporters.

Keywords: Transporters; Drug transport; Silencer RNA; Gene silencing; siRNA; Gene knockdown

SHORT INTEFERING RNA (siRNA) AS A NOVEL THERAPEUTIC

http://www.blackwell-synergy.com.libproxy1.nus.edu.sg/doi/abs/10.1111/j.1440-1681.2006.04399.x

SUMMARY
1. RNA interference (RNAi) is a robust method of post-transcriptional silencing of genes using double-stranded RNA (dsRNA) with sequence homology driven specificity. The dsRNA can be between 21 and 23 nucleotides long: this is converted to small interfering RNA (siRNA), which then mediates gene silencing by degradation/blocking of translation of the target mRNA.

2. RNA interference provides a simple, fast and cost-effective alternative to existing gene targeting approaches both in vitro and in vivo. The discovery of siRNAs that cause RNAi in mammalian cells opened the door to the therapeutic use of siRNAs. Highly intense research efforts are now aimed at developing siRNAs for therapeutic purposes.

3. Recent advances in the design and delivery of targeting molecules now allow efficient and highly specific gene silencing in mammalian systems. Synthetic siRNA libraries targeting thousands of mammalian genes are publicly available for high-throughput genetic screens for target discovery and validation. Recent studies have demonstrated the clinical potential of aptly designed siRNAs in various types of viral infections, cancer and renal and neurodegenerative disorders.

4. The present review provides insight into the novel therapeutic strategies of siRNA technology, which is the latest development in nucleic acid-based tools for knocking down gene expression, and its potential for silencing genes associated with various human diseases.